Journal: Nature Communications

Article Title: Hypoxia-driven remodeling of SELENOP + macrophages shapes T cell dynamics and promotes ovarian cancer metastasis

doi: 10.1038/s41467-025-67859-2

Figure Lengend Snippet: a The line plot showing the changes of relative fractions among macrophage clusters across the EAT, LAT and Met groups, p = 2.2 × 10 −16 , two-sided chi-squared test. Representative examples of ovarian tumor stained by multiplex immunohistochemistry, scale bar, 50 µm ( b ) and the quantification plots ( c ). For c , data are represented as means ± SD. Two-sided Kruskal–Wallis test, followed by Dunn’s post hoc test with adjustment. n = 20 samples from 11 HGSOC patients, including n =  5 EAT, n =  5 LAT, n =  5 Met. Ome, and n =  5 Met. Per, biological replicates. d Developmental trajectory of M05, M06 and M09 inferred by Monocle 2 analysis, color-coded by cluster (upper) and state (lower). Each dot represents a single cell. e Pathway enrichment analysis of the differential genes of cells in different state. Curve plots showing expression changes of function genes related to interferon signaling ( f , left), SELENOP expression ( f , right), HIF-1 signaling pathway ( g , left), and SPP1 expression ( g , right) along two cell fates. h Rank for regulons in SELENOP + macrophages and SPP1 + macrophages based on regulon specificity score (RSS). i Heatmap showing transcription factors (TF) activity for SELENOP + macrophages and SPP1 + macrophages. j Heatmap showing the RNA expression of TF along the pseudotime trajectory. k Western blot images (left) and quantification (right) of the levels of SELENOP proteins in THP-1 cells under control conditions or after treatment with 70 nM Se, 40 ng/mL IFNγ, or their combination. Se: Na 2 O 3 Se. l ELISA showing SELENOP level in the culture supernatants in ( k ). For k (right), l , data represent the mean ± SD. One-way ANOVA with Bonferroni post hoc test, n = 3, biological replicates. m Violin plots showing SELENOP expression of SELENOP + macrophages across the EAT, LAT and Met groups, colored by mean expression. Box of violin plot represents median ± interquartile range, the whiskers extend up to the minimum and maximum values. p values are calculated by two-sided Wilcoxon tests, adjusted by the Benjamini–Hochberg procedure. For a , d – j , m , n =  17 scRNA-seq cohort solid site samples, biological replicates. Source data are provided as a Source Data file.

Article Snippet: THP-1 cells were pretreatment with 70 nM Na 2 O 3 Se (Cat#HY-W686381, MedChemExpress) or equal volume PBS for 72 h, followed by stimulated with 40 ng/mL recombinant human IFNγ protein (Cat#C014, Novoprotein) or equal volume PBS for 48 h. BMDMs/MDMs were also stimulated with 40 ng/mL recombinant mouse/human IFNγ protein (Cat#C746, Cat#C014, Novoprotein) or equal volume PBS for 48 h. SELENOP expression was detected by extracting cellular proteins for Western blot, while SELENOP secretion was measured from collected cell culture supernatants.

Techniques: Staining, Multiplex Assay, Immunohistochemistry, Expressing, Activity Assay, RNA Expression, Western Blot, Control, Enzyme-linked Immunosorbent Assay